Journal: Scientific Reports

Article Title: Tectorigenin induces vasorelaxation in porcine coronary arteries through activation of Kv channels and oestrogen receptor modulation

doi: 10.1038/s41598-025-20988-6

Figure Lengend Snippet: Effects of neural and cyclic nucleotide pathway inhibitors on tectorigenin-induced relaxation in porcine coronary arteries pre-contracted with 100 nM U46619. ( A ) Tetrodotoxin (TTX, 1 µM) and ω-conotoxin GVIA (CTX, 1 µM) had no significant effect on the relaxation induced by 30 µM tectorigenin ( p > 0.05, n = 4). ( B ) Pretreatment with rolipram (1 µM, a selective phosphodiesterase-4 inhibitor) or vardenafil (1 µM, a selective phosphodiesterase-5 inhibitor) did not significantly alter tectorigenin-induced vasorelaxation ( p > 0.05, n = 4). ( C ) Inhibitors of the nitric oxide and cyclic nucleotide pathways, including Nω-nitro-L-arginine (L-NNA, 100 µM), KT5720 (1 µM, a PKA inhibitor), and KT5823 (1 µM, a PKG inhibitor), also did not significantly affect the relaxant response to tectorigenin ( p > 0.05, n = 4). Data are expressed as mean ± standard error of the mean (SEM) from four independent hearts. U46619 plateau (normalised to 60 mM KCl) was similar across groups ( p > 0.05; Supplementary Table 2).

Article Snippet: For experimental assays, a range of pharmacological agents was utilised, including U46619, apamin, KT5720, KT5823, and L-NNA (Sigma-Aldrich, MO, USA); rolipram, vardenafil, and TEA (Santa Cruz Biotechnology, CA, USA); IbTX (Alomone Labs, Jerusalem, Israel); glibenclamide (Research Biochemicals International, MA, USA); TTX and 4-AP (Tocris Bioscience, Bristol, UK); CTX (Bachem, Bubendorf, Switzerland); and charybdotoxin, methyl-piperidino-pyrazole (MPP), and PHTPP (Cayman Chemical, MI, USA).

Techniques:

Journal: Scientific Reports

Article Title: Tectorigenin induces vasorelaxation in porcine coronary arteries through activation of Kv channels and oestrogen receptor modulation

doi: 10.1038/s41598-025-20988-6

Figure Lengend Snippet: Effects of neural and cyclic nucleotide pathway inhibitors on tectorigenin-induced relaxation in porcine coronary arteries pre-contracted with 100 nM U46619. ( A ) Tetrodotoxin (TTX, 1 µM) and ω-conotoxin GVIA (CTX, 1 µM) had no significant effect on the relaxation induced by 30 µM tectorigenin ( p > 0.05, n = 4). ( B ) Pretreatment with rolipram (1 µM, a selective phosphodiesterase-4 inhibitor) or vardenafil (1 µM, a selective phosphodiesterase-5 inhibitor) did not significantly alter tectorigenin-induced vasorelaxation ( p > 0.05, n = 4). ( C ) Inhibitors of the nitric oxide and cyclic nucleotide pathways, including Nω-nitro-L-arginine (L-NNA, 100 µM), KT5720 (1 µM, a PKA inhibitor), and KT5823 (1 µM, a PKG inhibitor), also did not significantly affect the relaxant response to tectorigenin ( p > 0.05, n = 4). Data are expressed as mean ± standard error of the mean (SEM) from four independent hearts. U46619 plateau (normalised to 60 mM KCl) was similar across groups ( p > 0.05; Supplementary Table 2).

Article Snippet: For experimental assays, a range of pharmacological agents was utilised, including U46619, apamin, KT5720, KT5823, and L-NNA (Sigma-Aldrich, MO, USA); rolipram, vardenafil, and TEA (Santa Cruz Biotechnology, CA, USA); IbTX (Alomone Labs, Jerusalem, Israel); glibenclamide (Research Biochemicals International, MA, USA); TTX and 4-AP (Tocris Bioscience, Bristol, UK); CTX (Bachem, Bubendorf, Switzerland); and charybdotoxin, methyl-piperidino-pyrazole (MPP), and PHTPP (Cayman Chemical, MI, USA).

Techniques: